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KMID : 0364820140500040381
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Korean Journal of Microbiology
2014 Volume.50 No. 4 p.381 ~ p.383
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Isolation and Characterization of α-Glucosidase Inhibitor Produced by Bacillus sp. SKU31-1 Strain
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Kim Shin-Duk
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Abstract
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In the course of screening for α-glucosidase inhibitor produced by microorganism, the active compound was isolated
from the culture filtrate of Bacillus sp. SKU31-1 using a series of chromatography procedures. The structure of the
active compound was elucidated as 5-amino-1-hydroxymethyl-1, 2, 3, 4-cyclohexanetetrol on the basis of spectroscopic
evidence obtained and comparison with data from the literature. The active compound showed potent inhibitory
activity against α-glucosidase with an IC50 value of 1.9 μM for maltose and 4.9 mM for sucrose. A Lineweaver-Burk
plot indicated that its inhibition of α-glucosidase was competitive, with a Ki value of 0.15 mM.
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KEYWORD
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Bacillus sp., competitive inhibitor, α-glucosidase inhibitor, valiolamine
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